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pet28mkh8sumo vector  (Addgene inc)


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    Structured Review

    Addgene inc pet28mkh8sumo vector
    Pet28mkh8sumo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28mkh8sumo+vector/pET28-MKH8SUMO+(Plasmid+%2379526)/pm40082426-248-20-22
    Average 93 stars, based on 14 article reviews
    pet28mkh8sumo vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of a non-inhibitory aptameric ligand to CRL2 ZYG11B E3 ligase for targeted protein degradation.
    Article Snippet: .. The genes encoding human ZYG11B segments (amino acid residues 445-728) and ZER1 (amino acid residues 520–766) were cloned into the pET28MKH8SUMO vector (Addgene, #79526). ..

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Expressing:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Article Title: An engineered DNA aptamer-based PROTAC for precise therapy of p53-R175H hotspot mutant-driven cancer.
    Article Snippet: .. The bacterial expression plasmids for the DNA-binding domains of p53-WT and p53-R175H (aa: 94–293) were constructed by inserting the corresponding TP53 cDNA into the pET28MKH8SUMO vector (Addgene, Watertown, MA, USA). .. The Hisp53-WT and His-p53-R175H fusion proteins were expressed in Escherichia coli BL21 (DE3) strain and purified using a nickelcharged nitrilotriacetic acid (Ni-NTA) agarose column according to the manufacturer’s instructions (GE Healthcare, Pittsburgh, PA, USA).

    Purification:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Amplification:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Polymerase Chain Reaction:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    cDNA Library Assay:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Plasmid Preparation:

    Article Title: Molecular insights into degron recognition by CRL5 ASB7 ubiquitin ligase.
    Article Snippet: .. Protein expression and purification The human ASB7 (residues 11–318) was amplified via PCR from a human cDNA library and seamlessly cloned into the pET28MKH8SUMO vector (Addgene plasmid #79526). ..

    Construct:

    Article Title: An engineered DNA aptamer-based PROTAC for precise therapy of p53-R175H hotspot mutant-driven cancer.
    Article Snippet: .. The bacterial expression plasmids for the DNA-binding domains of p53-WT and p53-R175H (aa: 94–293) were constructed by inserting the corresponding TP53 cDNA into the pET28MKH8SUMO vector (Addgene, Watertown, MA, USA). .. The Hisp53-WT and His-p53-R175H fusion proteins were expressed in Escherichia coli BL21 (DE3) strain and purified using a nickelcharged nitrilotriacetic acid (Ni-NTA) agarose column according to the manufacturer’s instructions (GE Healthcare, Pittsburgh, PA, USA).



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    93
    Addgene inc pet28mkh8sumo vector
    Pet28mkh8sumo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28mkh8sumo+vector/pET28-MKH8SUMO+(Plasmid+%2379526)/pm40082426-248-20-22
    Average 93 stars, based on 1 article reviews
    pet28mkh8sumo vector - by Bioz Stars, 2026-09
    93/100 stars
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